Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna McGregor, Maya Anjur-Dietrich, and Kay Hung
Date created: 2012-12-07 12:00:00
Date modified: 2015-05-08 01:14:05
Calcium Induced Promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_M36543_sequence (Version 1) |
Description
This part is the promoter of the human prolactin (PRL) gene, which has been shown to be activated by increased levels of Ca2+ in cells. As there is little conclusive information about mammalian promoters other than they can be found in genetic control regions (usually upstream of the target gene), the genetic sequence was taken from the 2000 bases upstream of PRL in the human genome. Since this selection included a TATAA box, indicative of promoters that initiate at specific sites, it was possible to infer the presence of the PRL promoter.Notes
There were no changes to the original sequence, even for optimization purposes or to minimize repeats, due to the lack of information about mammalian promoters. This meant that any codon optimization could affect an area critical to the function of the promoter.Source
The part comes from the 2000 base pairs upstream of the human prolactin (PRL) gene.| Sequence Annotation | Location | Component / Role(s) |
| TATAA Box (Characteristic of a Promoter) PRL Promoter Region | 302,306 1,1998 | sequence_feature feature/misc feature/promoter promoter |