Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danielle Fraga, Jason Yang, Kaelo Moahi
Date created: 2013-12-06 12:00:00
Date modified: 2015-05-08 01:14:05
cAMP sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36547_sequence (Version 1) |
Description
This sensor should detect cAMP in media, activate cAMP regulator protein, and produce a PoPS signal, which then could be the input for an actuator, such as a Gemini reporter.Notes
The design behind using both a weak promoter and a weak RBS is intended to keep the E. coli alive since it is known that excessive concentrations of cAMP can be toxic to the cells. However, our second promoter is strong in order to increase our PoPS signal and give high expression of the actuator output.Source
The promoter, RBS, and terminator were sourced from Parts Registry.???BIOFAB Promoter Data: apFAB49.??? BIOFAB, n.d. Web. 24 Oct. 2013.
Mutalik, et. al. ???Precise and reliable gene expression via standard transcription and translation initiation elements.??? Figure 4C. Nature America. 2012. Web. 24 Oct. 2013.
"BIOFAB Promoter Data: apFAB47." BIOFAB, n.d. Web. 24 Oct. 2013.
The CRP coding sequence was sourced from UniProtKB.
???P0ACJ8 (CRP_ECOLI) Reviewed.??? Protein Knowledgebase(UniProtKB). UniProtKB/Swiss-Prot, n.d. Web. 25 Oct. 2013.
???Sequence Analysis.??? Reverse Translate. The Sequence Manipulation Suite, n.d. Web. 25 Oct. 2013.
| Access | Instance | Definition |
| public public public public public | BBa_M36541 BBa_M36542 BBa_M36544 BBa_M36545 BBa_M36546 | BBa_M36541 BBa_M36542 BBa_M36544 BBa_M36545 BBa_M36546 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36541 BBa_M36542 BBa_M36544 BBa_M36545 BBa_M36546 | 1,47 48,135 136,765 766,854 855,904 | BBa_M36541 BBa_M36542 BBa_M36544 BBa_M36545 BBa_M36546 |