Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong
Date created: 2015-10-21 11:00:00
Date modified: 2015-12-07 03:51:49
OYE1 Composite Sequence
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36552_sequence (Version 1) |
Description
Consists of multiple parts to produce the Old Yellow Enzyme 1 (OYE1) to reduce alpha-beta unsaturated C=C double bonds. Specifically consists of a rhamnose-inducible promoter (P_rha_BAD), a strong RBS, the OYE1 coding sequence, a His_* tag, and a termination sequence from T7.Notes
This design was done using a DNA2.0 software and GeneDesigner2.0 quality checks. The recommended design (in terms of promoters, RBS, tags, and terminators) was taken without amendments.Source
All of these are basic parts that have each of their sources documented individually.| Access | Instance | Definition |
| public public public public public | BBa_M36933 BBa_M36771 BBa_M36550 BBa_M36084 BBa_M36935 | BBa_M36933 BBa_M36771 BBa_M36550 BBa_M36084 BBa_M36935 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36933 BBa_M36771 BBa_M36550 BBa_M36084 BBa_M36935 | 1,114 115,127 128,1180 1181,1201 1202,1292 | BBa_M36933 BBa_M36771 BBa_M36550 BBa_M36084 BBa_M36935 |