Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christian Castaneda, Thai Nguyen, Andrew Rodriguez
Date created: 2012-12-06 12:00:00
Date modified: 2015-05-08 01:14:05
Ferredoxin-producing gene with iron sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36555_sequence (Version 1) |
Description
The ferredoxin, which is an iron-sulfur protein, is constitutively produced at high levels. Ferredoxin sequesters iron, so an iron sensor in the form of an iron-inducible promoter is put downstream of the ferredoxin gene. It may be possible to calculate the amount of ferredoxin produced by controlling the dissolved iron concentration and measuring the output of a reporter put downstream of the iron sensor. Comparing this result to a positive control of the reporter output of cultures with only the iron sensor would enable the calculation (part BBa_I716002).Notes
Optimized codons, G/C content, and repeats to 10 base pairs for S. elongatus.Source
http://partsregistry.org/Part:BBa_J23119; http://partsregistry.org/Part:BBa_B0034; http://www.ncbi.nlm.nih.gov/gene/4260838; http://partsregistry.org/Part:BBa_B0015; http://partsregistry.org/Part:BBa_I716002| Access | Instance | Definition |
| public public public public public | BBa_J23119 BBa_B0034 BBa_M36923 BBa_B0015 BBa_I716002 | BBa_J23119 BBa_B0034 BBa_M36923 BBa_B0015 BBa_I716002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0034 BBa_M36923 BBa_B0015 BBa_I716002 | 1,35 44,55 62,355 364,492 499,904 | BBa_J23119 BBa_B0034 BBa_M36923 BBa_B0015 BBa_I716002 |