BBa_M36576

BBa_M36576 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36576
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elisa Vidales
Date created: 2015-10-21 11:00:00
Date modified: 2015-10-22 10:25:01

qsc102 promoter + Whiteley Spacing



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_M36576_sequence (Version 1)

Description

Palindromic binding sequence and spacing for LasR bound to Pseudomonas Autoinducer.

Notes

Added 11 preceding base pairs to qsc102 palindromic binding sequence in order to optimize LasR complex binding.

Source

qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/
Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/

Sequence Annotation Location Component / Role(s)
LasR binding sequence
Binding Spacing 1
Whiteley Spacing
Transcription Start Site
12,31
1,11
32,64
65,65
feature/binding non_covalent_binding_site
feature/misc sequence_feature
feature/misc sequence_feature
feature/start start_codon
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M36576/1