Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katie Lund and Wyatt Woodson
Date created: 2011-04-25 11:00:00
Date modified: 2015-05-08 01:14:06
Pyruvate Sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36708_sequence (Version 1) |
Description
IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher.Notes
a lotSource
Katie Lund and Wyatt Woodsonparts obtained from Stanford BIOE44 - S11
| Access | Instance | Definition |
| public public public public public | BBa_J23119 BBa_M36001 BBa_M36706 BBa_M36010 BBa_M36707 | BBa_J23119 BBa_M36001 BBa_M36706 BBa_M36010 BBa_M36707 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_M36001 BBa_M36706 BBa_M36010 BBa_M36707 | 1,35 36,82 83,907 908,989 990,1131 | BBa_J23119 BBa_M36001 BBa_M36706 BBa_M36010 BBa_M36707 |