| Types | DnaRegion
|
| Roles | ribosome_entry_site
RBS
|
| Sequences | BBa_M36724_sequence (Version 1)
|
Description
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
Notes
Vivek says, "This is (the) Makoff design, it gives good expression and is seq independent of the Schoner design."
Source
Vivek Mutalik et al. c/o BIOFAB Emeryville.
