Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ray Paul Laureano-Mercado & Mark Kirollos
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:06
Melamine deaminase coding sequence
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M36761_sequence (Version 1) |
Description
This part is the coding sequence for the enzyme melamine deaminase, optimized for usage in E. coli. This enzyme eliminates an amine group from melamine to produce ammonia and ammeline and is able to remove another amine group at a rate 10 times slower than the first reaction to bring the compound to ammelide. The enzyme does not react to a measurable extent with this last compound, so it metabolizes melamine by consecutive and predictable deaminations.Notes
The TriA gene???s codons were modified from the original and optimized for E. coli to a Codon Adaptation Index (CAI) of .76. After that, some palindromic and mRNA destabilizing sequences were removed to increase the stability of our DNA during synthesis.Source
The original gene coding for the protein of interest was isolated from a species of Pseudomonas (http://www.ncbi.nlm.nih.gov/nuccore/11890744).| Sequence Annotation | Location | Component / Role(s) |
| Melamine Deaminase Start (ATG) Stop (TAG) | 1,1422 1,3 1423,1425 | feature/protein CDS start_codon feature/start stop_codon feature/stop |