Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Lee, Clinton Olivas, Daniel Hu
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:14:06
Chicken Lysozyme C (LYZ) conjugated with Albumin Signal Peptide
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M36762_sequence (Version 1) |
Description
Our part can be transformed into yeast for the production of mammalian lysozyme. Chicken lysozyme (LysA) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.Notes
We checked to make sure the sequence did not include any BsaI restriction sites. Given that the signal peptide is derived from mammalian cells, it is uncertain whether or not the yeast will read the peptide and secrete the lysozyme properly from the cytosol, despite it being yeast optimized.Source
The gene comes from iGem Part:BBa_K284001, a chicken lysozyme LysA gene.The signal peptide comes from Oxford Genetics optimized yeast lysozyme secretion vector, pSF-TEF1-NH2-Lys.