Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Lee
Date created: 2014-10-22 11:00:00
Date modified: 2015-05-08 01:14:06
Yeast-Optimized Chicken Lysozyme (LysA) Secretor
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M36763_sequence (Version 1) |
Description
Our device can be transformed into yeast for the production of mammalian lysozyme. It consists of a GAL1 promoter, the LysA gene linked to a yeast-optimized albumin signal peptide, and a Cyc1 terminator. Chicken lysozyme (LysA) has been shown to have high catalytic activity against gram-positive bacteria, hydrolyzing the peptidoglycan in the bacterial cell wall. The albumin signal peptide is optimized for lytic enzyme secretion in yeast expression hosts. By granting ethanologens, like yeast, the ability to secrete lysozyme, this part can be used to simultaneously decontaminate broths while carrying out fermentation.Notes
Since this is going to be transformed into yeast, the promoter and terminator have to be from yeast.Source
The GAL1 promoter comes from iGem parts registry: BBa_J63006The LysA gene comes from iGem parts registry: BBa_M36762
The Cyc1 terminator comes from iGem parts registry: Ba_K1462070
| Access | Instance | Definition |
| public public public | BBa_J63006 BBa_M36762 BBa_K1462070 | GAL1 BBa_M36762 BBa_K1462070 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J63006 BBa_M36762 BBa_K1462070 | 1,549 550,1088 1089,1338 | GAL1 BBa_M36762 BBa_K1462070 |