Types | DnaRegion
|
Roles | promoter
Regulatory
|
Sequences | BBa_M36801_sequence (Version 1)
|
Description
LacUV5 is a constitutive promoter mutated from of the lac promoter found in E. coli. The lac promoter is considered weak, it varies from the consensus sequence by 3 bases. On the other hand, the lacUV5 mutated promoter varies from the consensus sequence by only 1 base and is much stronger than the lac promoter. Even though lacUV5 is considered strong relative to other E. coli promoters, these are still weak when compared to T7 promoters.
Notes
No significant design issues encountered.
Source
http://www.biochem.wisc.edu/faculty/reznikoff/publications/139.pdf
http://openwetware.org/images/1/1c/TheBacterialPromoter.pdf