Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leah Chase
Date created: 2014-10-21 11:00:00
Date modified: 2015-05-08 01:14:06
Zinc Sensor
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M36827_sequence (Version 1) |
Description
This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.Notes
We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.Source
The genomic sequence comes from an E. Coli protein (ZntR) found in basic parts within the registry.| Access | Instance | Definition |
| public public public public | BBa_K190022 BBa_M11082 BBa_B0010 BBa_K190016 | BBa_K190022 BBa_M11082 BBa_B0010 BBa_K190016 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K190022 BBa_M11082 BBa_B0010 BBa_K190016 | 1,91 98,511 520,599 608,676 | BBa_K190022 BBa_M11082 BBa_B0010 BBa_K190016 |