Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leah Chase
Date created: 2014-10-21 11:00:00
Date modified: 2015-05-08 01:14:06
Zinc Sensor
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36828_sequence (Version 1) |
Description
This composite part contains a promoter and ribosome-binding-site, a gene for ZntR, a terminator, and a promoter for ZntA. In the absence of Zinc, the ZntR protein binds to the ZntA binding site. In the presence of zinc, ZntR undergoes a conformation, does not attach to the binding site, and allows for transcription of ZntA. Essentially, this part acts as a sensor for the presence of zinc.Notes
We had to enter silent mutations to change the DNA sequence without affecting the amino acid sequence because there were palindromes greater than 10 bp in every basic part.Source
The genomic sequence comes from an E. Coli protein (ZntR) found in basic parts within the registry.| Access | Instance | Definition |
| public public public public public | BBa_J23108 BBa_J61101 BBa_M36919 BBa_B0010 BBa_K190016 | BBa_J23108 BBa_J61101 BBa_M36919 BBa_B0010 BBa_K190016 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23108 BBa_J61101 BBa_M36919 BBa_B0010 BBa_K190016 | 1,35 36,47 48,461 462,541 542,610 | BBa_J23108 BBa_J61101 BBa_M36919 BBa_B0010 BBa_K190016 |