Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jasmine Johnson, Katie Plummer, Matthew Ryan Frankel
Date created: 2015-10-24 11:00:00
Date modified: 2015-10-25 02:02:24
p14ARF enhancer and promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_M36844_sequence (Version 1) |
Description
This sensor includes the p14ARF enhancer and promoter regions. Many oncogenes, including E2F1 and myc, are attracted to the p14ARF enhancer and bind to the promoter, initiating transcription of the p14aARF gene. p14ARF is an alternate reading frame protein that can activate p53, inducing apoptosis. This sensor allows for the detection of p14ARF expression by inserting the p14ARF promoter and enhancer into a S. cerevisiae sensor test rig (part BBa_M36093) or a mammalian sensor test rig (part BBa_M36097), both of which contain GFP coding sequences. Thus, cells transcribing the p14ARF enhancer and promoter sequence will be detectable through a Fluorescent Report Assay.Notes
This part requires insertion into a plasmid that expresses GFP or another fluorescent protein to allow for detection of transcription.Source
Homo sapiens p14ARF enhancer and promoter sequences| Sequence Annotation | Location | Component / Role(s) |
| p14ARF Enhancer p14ARF Promoter | 1,406 407,566 | sequence_feature feature/misc feature/misc sequence_feature |