Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alexandra Ritchie and Scott Platshon
Date created: 2013-06-09 11:00:00
Date modified: 2015-05-08 01:14:07
Beta-glucosidase actuator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36928_sequence (Version 1) |
Description
This device combines a medium strength RBS, a coding sequence for the beta-glucosidase enzyme, and a strong terminator (99% efficiency). It takes an input of PoPS and produces a translated enzyme BglA1 as an output.Notes
The medium strength RBS was chosen so as to translate enough protein but not too much to have negative impacts in the cells. The strong terminator was chosen to ensure that transcription stopped at the location we wanted. The RBS and terminator were taken from the BioFab Registry, so they were standardized for functional and physical composition.Source
This part is a composite of BBa_M36282, BBa_M36235, and the genomic sequence for the BglA1 gene from Shewanella frigidimarina, which is identified as BBa_M36926.| Access | Instance | Definition |
| public public public | BBa_M36282 BBa_M36926 BBa_M36235 | BBa_M36282 BBa_M36926 BBa_M36235 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36282 BBa_M36926 BBa_M36235 | 1,88 97,1422 1431,1519 | BBa_M36282 BBa_M36926 BBa_M36235 |