BBa_M36989

BBa_M36989 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M36989
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-06-07 11:00:00
Date modified: 2015-05-08 01:14:07

Secreting Antifreeze Protein Correcting Forward Primer



    • Details

    Types
    DnaRegion

    Roles
    Primer

    primer

    Sequences BBa_M36989_sequence (Version 1)

    Description

    Spacer + Xba1 + 5???UTR (BBa_M36009) + added start codons (ATGAAA), followed by the next 18 codons in the signal peptide sequence from (BBa_M36981)

    Notes

    Restriction enzyme was added to allow a cutting site for cloning of the amplified DNA sequence.

    Source

    Designed off of 5'UTR (BBa_M36009) and Type II signal peptide (BBa_M36981)

    Sequence Annotation Location Component / Role(s)
    Spacer
    Xba1 Restriction binding site
    5'UTR (BBa_M36009)
    start
    Type II Signal Peptide (BBa_M36981)
    		1,6
    7,12
    13,59
    60,62
    60,83
    		feature/misc sequence_feature
    non_covalent_binding_site feature/binding
    feature/misc sequence_feature
    start_codon feature/start
    tag feature/tag

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_M36989/1
     

    Attachments

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