Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-04-27 11:00:00
Date modified: 2015-05-08 01:14:07
Type II Secreted Antifreeze Actuator in E. coli (5-Repeat)
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_M36995_sequence (Version 1) |
Description
5'UTR, signal peptide, non-glycolated antifreeze protein, and terminator that will as whole transcribe and secrete antifreeze protein outside the cell membrane.Notes
This uses the 5-Repeat segments of the antifreeze protein, which can be fluctuated and remain functioning. Studies have shown, however, that increasing the number of repeating units in the antifreeze protein boosts its ability to act as antifreeze.Source
Based on a protein from the white flounder, Pseudopleuronecta americanus.| Access | Instance | Definition |
| public public public public | BBa_M36009 BBa_M36726 BBa_M36999 BBa_M36010 | BBa_M36009 BBa_M36726 BBa_M36999 BBa_M36010 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M36009 BBa_M36726 BBa_M36999 BBa_M36010 | 1,47 56,121 130,330 339,420 | BBa_M36009 BBa_M36726 BBa_M36999 BBa_M36010 |