Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-04-27 11:00:00
Date modified: 2016-01-27 10:45:33
Non-Glycolated Antifreeze Protein (AFP) - 5-Repeated Segment
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M36999_sequence (Version 1) |
Description
The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 5 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.Notes
This specific sequence has the repeat 5 times. Studies show that increasing the number of repeats increases the antifreeze capabilities of the protein.Source
Comes from the white flounder, or Pseudopleuronecta americanus.| Sequence Annotation | Location | Component / Role(s) |
| C-linker start Modified Unit 0 (TASDAAAAAL) Unit 1 (TAANAAAAAAL) Unit 2 (TAANAAAAAAL) Unit 3 (TAANAAAAAAL) Unit 4 (TAANAAAAAAL) stop | 1,9 10,12 16,45 46,78 79,111 112,144 145,177 187,192 | sequence_feature feature/misc feature/start start_codon feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/stop stop_codon |