Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nathan Barnett, Meghan Bowler, Kian Torabian
Date created: 2011-04-27 11:00:00
Date modified: 2016-01-27 10:45:52
Non-Glycolated Antifreeze Protein (AFP) - 10-Repeated Segment
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_M36996_sequence (Version 1) |
Description
The standard non-glycolated antifreeze protein (AFP) from the Pseudopleuronecta americanus. Begins with a chain of C's and is followed by 10 rough repeats of "ANAAAAAALTA". The codons have been optimized for E. coli.Notes
This sequence has 10 rough repeats of "ANAAAAAALTA", as compared to the natural peptide which has this sequence repeated roughly 5 times.Source
Based on the 5-repeated Segment AFP from the white flounder, Pseudopleuronecta americanus.| Sequence Annotation | Location | Component / Role(s) |
| C - linker start Modified Unit 0 (TASDAAAAAL) Unit 1 (TAANAAAAAAL) Unit 2 (TAANAAAAAAL) Unit 3 (TAANAAAAAAL) Unit 4 (TAANAAAAAAL) Unit 5 (TAANAAAAAAL) Unit 6 (TAANAAAAAAL) Unit 7 (TAANAAAAAAL) Unit 8 (TAANAAAAAAL) Unit 9 (TAANAAAAAAL) stop | 1,9 10,12 16,45 46,78 79,111 112,144 145,177 178,210 211,243 244,276 277,309 310,342 355,360 | feature/misc sequence_feature feature/start start_codon sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature stop_codon feature/stop |