Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nick Davis, Rachel RoseFigura
Date created: 2011-04-27 11:00:00
Date modified: 2015-05-08 01:14:07
Coenzyme B-12 Riboswitch for E. coli (nucleotides 1-240)
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_M36998_sequence (Version 1) |
Description
This part contains the full string of nucleotides for the coenzyme B-12 riboswitch present in Escherichia coli. The aptamer domain of the riboswitch (nucleotides 1-202) undergoes substantial allosteric changes upon binding coenzyme B-12 (aka hydroxocobalamin). The coenzyme B-12 aptamer has one of the most complex metabolite-binding structures of known naturally-occurring riboswitches, though other riboswitches with simpler aptamers can also sense their targets with impressive precision and use the resultant structural changes to control gene expression.Nucleotides 203-240 represent the 5'UTR (untranslated region) upstream of the btuB coding sequence in E. coli genome.
Notes
An executive decision had to be made to include the 5'UTR region following the cobalamin ribowitch (aptamer) sequence. Nucleotides 203-240 represent the 5'UTR upstream of the btuB coding sequence. We chose to include these nucleotides in our part, as it is not completely clear what distinct role this region plays in translational control by the riboswitch, and few studies have demonstrated the significance of this region.Source
Naturally-occurring, 240-nucleotides upstream of the btuB (B-12 uptake) coding sequence in E. coli. BtuB (the protein coded by the btuB gene) is a B-12 membrane transporter in E. coli bacteria.| Sequence Annotation | Location | Component / Role(s) |
| Shine-Dalgarno Sequence B12 box/B12 element | 210,237 142,159 | ribosome_entry_site feature/rbs conserved_region feature/conserved |