Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edgardo Farias
Date created: 2012-05-11 11:00:00
Date modified: 2015-05-08 01:14:07
Glucose->Insulin
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M39123_sequence (Version 1) |
Description
This device is made from several different parts taken from E. coli and Yeast. Our goal in creating this device was to be able to measure glucose levels and have the cells then produce an appropriate amount of insulin in response. To do this, we used the following parts: CAP binding site from the Lac Operon (taken from E. coli.), the Promoter found in the Lac Operon, a yeast ribosome binding site, the cDNA for human insulin, and a yeast terminator. We plan to use this device in yeast. As glucose is taken in, levels of cAMP increase. cAMP binds to the CAP binding site, facilitating the binding of RNA polymerase to the promoter and allowing transcription of the insulin gene. As glucose levels rise, so does that of cAMP, increasing the amount of insulin produced. Once glucose levels lower, cAMP lowers, and there is less insulin produced.Notes
We had to figure out how to detect levels of glucose and respond to that accordingly. We solved this by looking at existing regulatory systems and modifying them to work in s cerevisiae.Source
CAP: E coliPromoter: E coli
RBS: s cerevisiae
Insulin: humans
Terminator: s cerevisiae
| Access | Instance | Definition |
| public public | BBa_K165002 BBa_J63002 | BBa_K165002 tADH1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K165002 BBa_J63002 | 1,18 27,251 | BBa_K165002 tADH1 |