Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hao Xing
Date created: 2013-05-11 11:00:00
Date modified: 2015-05-08 01:14:08
AlkS coding sequence in Pseudomonas putida
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_M41004_sequence (Version 1) |
Description
This gene encodes a transcription factor AlkS, which activates alkane degradation operon. AlkS self-regulates its expression. In the presence of alkanes, AlkS forms AlkS-hydrocarbon (AlkS-HC) complex which downregulates pAlkS1 and upregulates pAlkS2. AlkS-HC complex activates pAlkB which is responsible for expression of alkane degradation operon.Notes
We cannot separate pAlkS1, pAlkS2 and AlkS gene because of self-regulatory system.Source
Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9.Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30.