BBa_M41017

BBa_M41017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M41017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hao Xing

Date created: 2013-05-13 11:00:00

Date modified: 2015-05-08 01:14:08

This sequence encodes a 64-nucleotide RNAi.

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_M41017_sequence (Version 1)

Description

The RNAi (RdlD) sequence is the inhibitor of the toxin (IdrD), which blocks the translation of the toxin.

Notes

Take off the original promoter and fuse it with LacI sequence.

Source

Mitsuoki Kawano, Taku Oshima, Hiroaki Kasai, and Hirotada Mori. "Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli." Molecular Microbiology 45.2 (2002): 333???349. Web.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Poly A tail	1,4	feature/polya, polyA_site
RNAi coding sequence	5,68	CDS, feature/protein
Promoter	69,99	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M41017/1

Member of these Collections

• iGEM Parts Registry