BBa_M45689

BBa_M45689 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M45689
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Spencer Williams
Date created: 2015-04-13 11:00:00
Date modified: 2015-05-08 01:14:09

Constitutive promoter with a retinoic acid response element at the end



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_M45689_sequence (Version 1)

Description

This promoter is designed to constantly be on except in the presence of both the retinoic acid receptor protein and retinoic acid.

Notes

Had to considered whether the response element would interfere with the promoter activity with no retinoic acid present.

Source

The promoter used is from the LacIQ promoter from E. coli, and the retinoic acid response element(RARE) from the homo sapien genome.

Sequence Annotation Location Component / Role(s)
-35 Box
-10 Box
RARE
3,8
24,30
54,71
promoter feature/promoter
promoter feature/promoter
feature/binding non_covalent_binding_site
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M45689/1