BBa_M45802

BBa_M45802 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M45802

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Thomas Overbeck

Date created: 2015-04-14 11:00:00

Date modified: 2015-05-08 01:14:09

Mutated Tuc2009 bidirectional promoter for decreased CI sensitivity

Details

• Types: DnaRegion
• Roles: T7_RNA_Polymerase_Promoter; T7
• Sequences: BBa_M45802_sequence (Version 1)

Description

A modified bi-directional promoter from lactococcal bacteriophage Tuc2009 containing a bp mutation for decreased binding affinity of the CI repressor protein to the operator OL-CI at high CI concentrations. Allowing for continued up-regulated transcription from the leftward promoter (PL). See BBa_M45801 for detailed description of bi-directional promoter and intergenic switch region regulation with CI and Cro repressor proteins.

Notes

G to T change at bp 32 from BBa_M45801 on Fwd strand

Source

Kenny JG, Leach S, Hoz AB, Venema G, Kok J, Fitzgerald GF, Nauta S, Alonso JC, Sinderen D (2006) Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009. Virology 347:434-446

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
G to T	32,32	sequence_alteration, feature/mutation
PL	7,36	feature/promoter, promoter
PR	64,92	promoter, feature/promoter
OL-CRO	5,33	feature/operator, operator
OR-CRO	40,78	feature/misc, sequence_feature
OL-CI	17,44	operator, feature/operator
OR-CI	65,92	feature/operator, operator

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M45802/1

Member of these Collections

• iGEM Parts Registry