BBa_M47008

BBa_M47008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M47008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julian Alverio
Date created: 2015-05-12 11:00:00
Date modified: 2015-05-12 11:33:56

Time-Delay Input Filter



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_M47008_sequence (Version 1)

Description

The first component in this part is an inducible promoter. This acts as the receiver for the input signal and can be modified according to the need of a system. In the sequence submitted, this is the Lac Operon Promoter. The promoter regulates the transcription of a weak inducer protein that must homodimerize in order to become active. The need for homodimerization introduces the time delay as the protein needs to build up for an extended period of time before becoming active. The exact protein chosen for this component can be varied depending on the delay needed. In the sequence submitted, this is HD-Zip family transcription factor Hat4. This transcription factor is followed by a transcriptional terminator sequence and then the promoter which our transcription factor activates; in this case COX5B-1. This secondary promoter directly regulates the part's output. This output is modular but in the submitted sequence it is GFP.

Notes

Strength of interaction between the homodimerizing inducer and its promoter will determine how long the output lags the input. Multiple combinations will likely need to be tested in order to achieve desired performance.

Source

Lac Operon Promoter from E. Coli Genome
Hat4 gene and COX5B-1 promoter sequence from Arabidopsis thalian genome
GFP from Aequorea victoria genome

Sequence Annotation Location Component / Role(s)
Lac Operon Promoter
Hat4
Transcriptional Terminator
COX5B-1
GFP
1,37
47,913
914,960
961,1851
1862,2551
feature/promoter promoter
CDS feature/protein
feature/stop stop_codon
promoter feature/promoter
CDS feature/protein
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M47008/1