Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julian Alverio
Date created: 2015-05-12 11:00:00
Date modified: 2015-05-12 11:33:56
Time-Delay Input Filter
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_M47008_sequence (Version 1) |
Description
The first component in this part is an inducible promoter. This acts as the receiver for the input signal and can be modified according to the need of a system. In the sequence submitted, this is the Lac Operon Promoter. The promoter regulates the transcription of a weak inducer protein that must homodimerize in order to become active. The need for homodimerization introduces the time delay as the protein needs to build up for an extended period of time before becoming active. The exact protein chosen for this component can be varied depending on the delay needed. In the sequence submitted, this is HD-Zip family transcription factor Hat4. This transcription factor is followed by a transcriptional terminator sequence and then the promoter which our transcription factor activates; in this case COX5B-1. This secondary promoter directly regulates the part's output. This output is modular but in the submitted sequence it is GFP.Notes
Strength of interaction between the homodimerizing inducer and its promoter will determine how long the output lags the input. Multiple combinations will likely need to be tested in order to achieve desired performance.Source
Lac Operon Promoter from E. Coli GenomeHat4 gene and COX5B-1 promoter sequence from Arabidopsis thalian genome
GFP from Aequorea victoria genome
| Sequence Annotation | Location | Component / Role(s) |
| Lac Operon Promoter Hat4 Transcriptional Terminator COX5B-1 GFP | 1,37 47,913 914,960 961,1851 1862,2551 | feature/promoter promoter CDS feature/protein feature/stop stop_codon promoter feature/promoter CDS feature/protein |