eCPX-nSA-c

BBa_M50000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_M50000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicolas Quach
Date created: 2016-10-23 11:00:00
Date modified: 2016-12-09 07:58:12

Streptavidin binding outer membrane protein



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_M50000_sequence (Version 1)

Description

Cell surface display protein with N-terminal and C-terminal streptavidin binding domain. eCPX-nSA-cNano will be used to make E. coli bind to streptavidin coated magnetic beads, enabling bacterial capture.

Notes

eCPX was developed as a biterminal display scaffold, and thus both N and C termini are available for fusion with other domains. An N terminal fusion with a streptavidin binding domain had already been created by Rice and Daugherty for their paper on eCPX; we wished to increase the affinity by fusing another (stronger binding) streptavidin binding domain to the C terminus

Source

Derived from E. coli outer membrane protein OmpX. eCPX is an engineered version of OmpX developed by Rice and Daugherty (Protein Eng. Des. Sel., 2008, pg 435-442)

Sequence Annotation Location Component / Role(s)
NanoTag
SA-binding domain
Ser-Gly linker
635,679
86,130
617,634
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_M50000/1