Types | DnaRegion
|
Roles | Protein_Domain
polypeptide_domain
|
Sequences | BBa_M50000_sequence (Version 1)
|
Description
Cell surface display protein with N-terminal and C-terminal streptavidin binding domain. eCPX-nSA-cNano will be used to make E. coli bind to streptavidin coated magnetic beads, enabling bacterial capture.
Notes
eCPX was developed as a biterminal display scaffold, and thus both N and C termini are available for fusion with other domains. An N terminal fusion with a streptavidin binding domain had already been created by Rice and Daugherty for their paper on eCPX; we wished to increase the affinity by fusing another (stronger binding) streptavidin binding domain to the C terminus
Source
Derived from E. coli outer membrane protein OmpX. eCPX is an engineered version of OmpX developed by Rice and Daugherty (Protein Eng. Des. Sel., 2008, pg 435-442)