BBa_M50007

BBa_M50007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M50007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Cale Lester, Raquel Freeman, Tristan Yeung

Date created: 2016-10-31 12:00:00

Date modified: 2016-12-11 08:40:03

U1 Thermosensitive 5'-UTR (includes RBS)

Details

• Types: DnaRegion
• Roles: RBS; ribosome_entry_site
• Sequences: BBa_M50007_sequence (Version 1)

Description

It utilizes the distinct thermosensor U1, with different expression patterns at various temperature levels.

At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. We picked U1 because of its unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. We did not make any modifications to the thermosensor genetic sequences given by Neupert et. al.

Our plasmid includes a standard selectable marker, chloramphenicol, pP-T5 promoter, the U1 RBS, mTurquoise FP, pT-T7 terminator, and a high copy ORI.

Notes

Less than 2kb for DNA 2.0 to synthesize. No Bsa I restriction site sequence. No palindromic repeats longer than 5 bp or repeating nucleotides longer than approximately 10 bp.

Source

Our unique parts are based off of E. coli thermosensors designed by Neupert et. al.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M50007/1

Member of these Collections

• iGEM Parts Registry