Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cale Lester, Raquel Freeman, Tristan Yeung
Date created: 2016-10-31 12:00:00
Date modified: 2016-12-11 08:40:54
U10 Thermosensitive 5'-UTR (includes RBS)
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_M50008_sequence (Version 1) |
Description
U10 RBS, contains the Shine Dalgarno sequence and an anti-Shine-Dalgarno sequence which will form a hairpin structure in mRNA that inhibits ribosomes from binding to the mRNA to initiate translation. Normal protein synthesis at 37 degrees Celsius but from thirty degrees Celsius and below little to no protein is made.Notes
No BsaI sites, no palindromic repeats greater than 10bp, not over 2kbp.Source
Neupert, J., Karcher, D., & Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.Synthetically designed RBS site with internal hairpin for thermosensitivity.