Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Derek Wang, Anika Naidu
Date created: 2016-10-26 11:00:00
Date modified: 2016-12-01 12:19:13
Positive feedback cusR producer (E. coli)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_M50015_sequence (Version 1) |
Description
This sequence contains BsaI restriction enzyme sites on both ends. Following the BsaI restriction site on the 5' end, there is the promoter activated by cusR, followed by a high copy number RBS, followed by the sequence for cusR with STOP sequence at the end. Finally, there is the complementary BsaI restriction site.Notes
Designed to be grafted onto EColi_Promoter_Casette with high copy number RBS and GFPSource
RBS: BBa_B0035 http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_CollectioncusR promoter from E.Coli K12 (250 nts before cusR protein sequence): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4
cusR from E.Coli K12 (684 nts of protein): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4
| Access | Instance | Definition |
| public public public | BBa_M50014 BBa_B0035 BBa_M50022 | BBa_M50014 BBa_B0035 BBa_M50022 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_M50014 BBa_B0035 BBa_M50022 | 1,250 259,272 279,962 | BBa_M50014 BBa_B0035 BBa_M50022 |