BBa_M50016

BBa_M50016 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_M50016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Maurice Chiang

Date created: 2016-10-26 11:00:00

Date modified: 2016-10-27 07:34:50

Promoter, RBS and GolS Activator for Gold Detection

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_M50016_sequence (Version 1)

Description

Included in this composite part is a promoter, RBS, and GolS protein for gold detection. The GolS protein, in the presence of Au forms a metal-protein complex that then binds to the promoter as an activator, up-regulating downstream gene expression.

Notes

We inserted Bsal (FW) and Bsal (RV) for synthesis with the RBS for GFP and GFP in the overall plasmid. The sequence for the sensor and actuator for this device is: Bsal (FW)_Promoter_RBS_GolS_Bsal (RV)_RBS_GFP.

Source

This part was drawn from York iGEM in 2013, found at this link: http://parts.igem.org/Part:BBa_K1127008

Components

Access	Instance	Definition
public	BBa_M50020	BBa_M50020

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_M50020	1,38	BBa_M50020

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M50016/1

Member of these Collections

• iGEM Parts Registry