| Types | DnaRegion
|
| Roles | polypeptide_domain
Protein_Domain
|
| Sequences | BBa_M50036_sequence (Version 1)
|
Description
This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two GBP domains linked on each end to a flurophore. The linkers were included to ensure that glucose-binding caused the conformational change characterized with FRET instead of inherently folded protein structure, the linkers were included.
Notes
Codon optimization software was used since we transformed our plasmid into E. coli. Also, the linker needed to be relatively short since we were already designing a long construct and needed to minimize the synthesis time given class constraints.
Source
Linker sequence was suggested and provided by our TA, Salil Bahte, and then optimized for use in E. coli.
