BBa_M50048

BBa_M50048 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M50048

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eleanor Glockner, Jack Andraka

Date created: 2016-12-10 12:00:00

Date modified: 2016-12-11 11:22:31

Atrazine riboswitch

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_M50048_sequence (Version 1)

Description

This is a riboswitch consisting of an RNA aptamer that recognizes atrazine, and a ribosome binding site. It is based on BBa_K784005, with the theophylline aptamer exchanged for an atrazine aptamer. In the absence of atrazine, the RNA transcript of this part has excessive base pairing in the 5??? UTR, folding the RNA to block binding at the RBS and thus prevent translation. When atrazine is present, it binds to the aptamer sequence, altering the shape of the transcript to expose the RBS and allow translation of any downstream protein sequences.

Notes

We had to replace the aptamer in the original riboswitch with an aptamer that would target our molecule of interest.

Source

Riboswitch components: http://parts.igem.org/Part:BBa_K784005

Atrazine aptamer:
Williams, R., Crihfield, C., Gattu, S., Holland, L., & Sooter, L. (2014). In Vitro Selection of a
Single-Stranded DNA Molecular Recognition Element against Atrazine. IJMS International
Journal of Molecular Sciences, 15(8), 14332-14347. doi:10.3390/ijms150814332

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
5' UTR	1,17	sequence_feature, feature/misc
atrazine aptamer	18,90	sequence_feature, feature/misc
RBS	91,111	feature/rbs, ribosome_entry_site

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M50048/1

Member of these Collections

• iGEM Parts Registry