BBa_M50055

BBa_M50055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_M50055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eleanor Glockner

Date created: 2016-12-11 12:00:00

Date modified: 2016-12-12 12:34:30

Atrazine biosensor

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_M50055_sequence (Version 1)

Description

This is an atrazine biosensor that reflects the presence of atrazine through GFP fluorescence. A UTR in the riboswitch causes excessive base pairing in the RNA transcript, folding the RNA such that the RBS is not normally exposed. When atrazine is present, it binds to the aptamer sequence and alters the shape of the RNA to allow binding at the RBS, thus allowing translation of a GFP sequence following the RBS. The level of GFP expression corresponds to the level of atrazine in the surrounding environment.

Notes

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Source

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Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
5' UTR	1,17	sequence_feature, feature/misc
BBa_M50048	1,111	feature/BioBrick, engineered_region
atrazine aptamer	18,90	feature/misc, sequence_feature
RBS	91,111	feature/rbs, ribosome_entry_site
eGFP	112,842	CDS, feature/cds

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_M50055/1

Member of these Collections

• iGEM Parts Registry