Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leon Chan
Date created: 2004-07-27 11:00:00
Date modified: 2015-05-08 01:14:11
ccdB cell death gene
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_P1010_sequence (Version 1) |
Description
The ccd operon having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. This part is used to aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up. P1010 is only provided as plasmid or in DB3.1 which is ccdB resistant.| Sequence Annotation | Location | Component / Role(s) |
| ccdB (rev) ccdA- (rev) T to C mutation -10 (rev) -35 (rev) | 29,334 336,556 467,467 595,600 618,623 | feature/cds CDS CDS feature/cds feature/s_mutation point_mutation feature/misc sequence_feature feature/misc sequence_feature |