luxR

BBa_R0061 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0061
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
Date created: 2004-01-28 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (HSL-mediated luxR repressor)



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_R0061_sequence (Version 1)

    Description

    This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.

    Sequence Annotation Location Component / Role(s)
    -35
    -10
    Lux Box
    		1,6
    25,30
    6,25
    		feature/promoter promoter
    promoter feature/promoter
    non_covalent_binding_site feature/binding

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0061/1
     

    Attachments

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