cinR

BBa_R0077 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0077
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: crackdots
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (cinR and HSL regulated, RBS+)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R0077_sequence (Version 1)

    Description

    CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription.

    This promoter contains the RBS: TGGAGG

    Notes

    The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci

    Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ

    MOL. MICROBIOLOGY 37(1): 81-97, 2000

    Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.

    Source

    Rhizobium leguminosarum

    Sequence Annotation Location Component / Role(s)
    embedded RBS
    stem_loop
    stem_loop
    unidentified/uncharacterized CinR dependent
    		226,231
    35,74
    91,136
    1,220
    		feature/rbs ribosome_entry_site
    stem_loop feature/stem_loop
    feature/stem_loop stem_loop
    promoter feature/promoter

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0077/1
     

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