cI 434

BBa_R1052 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R1052
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maia Mahoney, Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter, Standard (434 cI regulated)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_R1052_sequence (Version 1)

Description

[Note: This is the same part as R0052 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, BBa_C0052, binds to it.
This segment contains O-R1, O-R2, P-R, and P-RM -35.



Notes

Wild-type promoter modified to comply with BBa_S0001:
TTGACA-17N-GATACT



Source

Bacteriophage 434 right operator

Sequence Annotation Location Component / Role(s)
-10
OR2
OR1
-35
-35
start
-10
BBa_R1052
1,43
8,21
30,43
1,6
2,7
35,37
24,29
1,46
feature/promoter promoter
feature/binding non_covalent_binding_site
non_covalent_binding_site feature/binding
feature/promoter promoter
feature/promoter promoter
start_codon feature/start
promoter feature/promoter
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R1052/1