cII p22

BBa_R1053 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R1053
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maia Mahoney, Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter, Standard (p22 cII regulated)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_R1053_sequence (Version 1)

Description

[Note: This is the same part as R0053 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it.
This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)



Notes

Modified from wild-type to comply with BBa_S0001:
TTGACA-17N-GATACT



Source

Bacteriophage p22.

Sequence Annotation Location Component / Role(s)
OR3
OR2
OR1
-35
-35
-10
BBa_R1053
1,3
11,29
35,52
19,24
8,14
31,36
1,55
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
promoter feature/promoter
feature/promoter promoter
promoter feature/promoter
feature/BioBrick engineered_region
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R1053/1