Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Heather Keller
Date created: 2007-03-05 12:00:00
Date modified: 2015-05-08 01:14:16
R0011+B0100+B0048
| Types | DnaRegion |
| Roles | engineered_region Intermediate |
| Sequences | BBa_S00159_sequence (Version 1) |
Description
This part is an intermediate assembly product that contains the R0011 promoter (lambda cI promoter with lacI binding sites), the OmpaA 5' UTR containing a double hairpin for transcript stability, and an AvrII restriction site to facilitate later downstream cloning of an RBS via Heather Keller's RBS characterization scheme. These parts are cloned bluntly, without generating biobricks mixed sites in between.Notes
This part was generated by PCR assembly in order to minimize the number of digestions and ligations necessary to generate a larger construct. Especially given the short sequence of the three individual parts (and the difficulting in purifying small parts) it was much simpler to amplify the product in this way rather than generate the 3 parts individually and clone them together.Source
This part was generated by PCR amplification. The upstream biobricks cut sites (EcoRI and XbaI) as well as the R0011 promoter were incorporated into the forward promoter recognizing the ompA region in the MG1655 genome. The AvrII cut site, as well as the downstream biobricks cut sites (SpeI and PstI) were incorporated into the reverse promoter.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 lac O1 -35 lac O1 -10 hp1 ss1 hp2 ss2 BBa_B0100 AvrII site BBa_B0048 | 1,54 3,19 20,25 26,42 43,48 56,118 119,129 130,158 159,170 56,170 171,176 171,176 | feature/BioBrick engineered_region operator feature/operator promoter feature/promoter feature/operator operator promoter feature/promoter feature/stem_loop stem_loop feature/misc sequence_feature feature/stem_loop stem_loop feature/misc sequence_feature engineered_region feature/BioBrick sequence_feature feature/misc engineered_region feature/BioBrick |