7x His

BBa_T2000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_T2000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2009-08-10 11:00:00
Date modified: 2015-05-08 01:14:52

7x His tag tail domain



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_T2000_sequence (Version 1)

Description

This is a 6x His tag tail domain designed to encode the C-terminus of a protein. His tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Notes

Used both His codons in order to avoid repeats. Also included a double TAATAA stop codon.

Source

No. This is a synthetic sequence.

Sequence Annotation Location Component / Role(s)
7x His
double stop codon
1,21
22,27
feature/tag tag
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_T2000/1