FLAG

BBa_T2005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_T2005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2009-08-10 11:00:00

Date modified: 2015-05-08 01:14:52

FLAG octapeptide special internal domain (DYKDDDDK)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_T2005_sequence (Version 1)

Description

This is a FLAG tag octapeptide special internal domain designed to be internal to the sequence of a protein. FLAG tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Notes

The FLAG sequence AspTyrLysAspAspAspAspLys (DYKDDDDK) is one of the most commonly used FLAG tag sequences. Each of the amino acids in this octapeptide have two possible codon choices. For each amino acid, neither of the two codon choices appear to be rare in the common model organisms. So codons were selected to minimize repeats.

Source

This is a synthetic sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FLAG octapeptide	1,24	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_T2005/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/internal/special
• iGEM Parts Registry