Strep-tag

BBa_T2014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_T2014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2009-08-12 11:00:00

Date modified: 2015-05-08 01:14:52

Strep-tag II special internal domain (WSHPQFEK)

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_T2014_sequence (Version 1)

Description

This is a Strep tag tail domain designed to be internal to a protein. Strep tags are typically used for detection, purification and immobilization of proteins.

Notes

This domain encodes the Strep tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

Source

This is a synthetic sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Strep-tag II	1,24	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_T2014/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/internal/special
• iGEM Parts Registry