Types | DnaRegion
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Roles | Cell
chromosome
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Description
Barry Wanner E. coli strain useful for the maintanence of plasmids with the R6K origin of relication and conjugal transfer of plasmids with an RP4 origin of transfer. These properties are conferred by the pir+ gene and a oriT-deficient copy of the RP4 plasmid.
Strain principly used as the donor strain for transposon mutagenesis.
Genotype: RP4-2-tet:Mu-1kan::Tn7 integrant leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 gusA (DMluI)::pir?? thi
Notes
N/A
Source
Barry Wanner