| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_Y00012_sequence (Version 1)
|
Description
S. cerevisiae gene LYS12 [
http://db.yeastgenome.org/cgi-bin/locus.pl?locus=lys12] is required for the fourth step of lysine biosynthesis. It is normally encoded by the nuclear genome and the protein is imported to the mitochondria where it oxidatively decarboxylates homo-isocitrate to alpha-ketoadipate. Strains deleted for LYS12 are viable but show decreased growth in the absence of lysine. Part Y00012 is the S. cerevisiae LYS12 gene that has been recoded for expression in the mitochondria, which uses a different genetic code and separate transcription/translation machinery for expression mitochondrial genes. The part is start to stop flanked by biobrick ends so has no promoter for expression or sequences for recombination into the mitochondria.
Notes
codon optimized for expression in S. cerevisiae mitochondria, keeping all biobrick sites out of coding region
Source
custom synthesis from DNA 2.0
