Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: T7.2
Date created: 2005-04-04 11:00:00
Date modified: 2015-05-08 01:14:54
T7.2 gene 0.7
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_Z0106_sequence (Version 1) |
Description
Gene 0.7 codes for a protein that inactivates host-catalyzed transcription, it is also a serine-threonine protein kinase. The two activities are separable. Gp0.7 phosphorylates many host proteins, including ribosome components, ribonucleases III and E, and the β and β?? subunits of E. coli RNAP. The BR3 and Y49 strains contain the rpoC-E1158K mutation, which renders gp0.7 essential for phage growth. The mutation affects the region of β?? to which the T7 protein gp2 binds. Gene 0.7 is non-essential in wild-type hosts and is actually detrimental when T7 is grown at 30˚C in rich media. Early T7 RNA and protein synthesis is prolonged after infection by gp0.7 mutants. However, phage growth at elevated temperatures or in minimal media, or in cells harboring the Col Ib plasmid renders gene 0.7 essential. The processes underlying the conditional necessity for gp0.7 function have not been determined.The E. coli C promoter -35 region is located on the 3' end of the coding sequence. Removing the downstream region will probably be sufficient to delete functioning of the promoter.
Source
T7 genome reannotation| Sequence Annotation | Location | Component / Role(s) |
| -35 region of C promoter -45 region of C promoter added BsiWI | 1058,1062 1049,1049 1081,1086 | feature/promoter promoter promoter feature/promoter feature/mutation sequence_alteration |