Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-10-23 11:00:00
Date modified: 2015-05-08 01:14:55
pUC19 with a BioBrick cloning site (Replaced by pSB1A3)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | pSB1A1_sequence (Version 1) |
Description
This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19.Notes
It is not entirely clear whether there are two contigous copies of EcoRI restriction site or one copy of the EcoRI in this vector. Sequence information did not include this section of the vector. In this part, only a single EcoRI restriction site is shown.Source
pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt| Sequence Annotation | Location | Component / Role(s) |
| M13Reverse priming site P(LAC) transcription start site P(LAC) -10 site P(LAC) -35 site CAP protein binding site Lac operon ORI RNAII transcript origin of replication RNAI transcript RNAI transcript promoter -35 site RNAI transcript promoter -35 site RNAII transcript promoter -10 site RNAII transcript promoter -35 site pBR322 beta-lactamase (bla; amp-r) beta-lactamase signal peptide bla RNA transcription start P(BLA) -10 site P(BLA) -35 site pBR322 M13 Forward priming site BioBricks suffix BioBricks prefix | 70,86 112,112 119,124 143,148 168,180 60,289 472,472 472,1024 472,1060 914,1021 878,883 900,906 1034,1039 1055,1060 290,2291 1231,2091 2023,2091 2126,2126 2135,2140 2156,2161 2292,2428 2670,2685 1,21 2687,2708 | primer_binding_site feature/primer_binding sequence_feature feature/misc feature/promoter promoter promoter feature/promoter feature/operator operator sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/promoter promoter promoter feature/promoter feature/promoter promoter feature/promoter promoter feature/misc sequence_feature feature/cds CDS feature/cds CDS feature/misc sequence_feature feature/promoter promoter feature/promoter promoter feature/misc sequence_feature feature/primer_binding primer_binding_site feature/misc sequence_feature sequence_feature feature/misc |