Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55
pSB1AC3 with ccdB insert
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | pSB1AC3_1_sequence (Version 1) |
Description
Target plasmid for three antibiotic assembly. Chloramphenicol resistant. Contains an insert having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. Grown in the special strain DB3.1, which is ccdB resistant.| Sequence Annotation | Location | Component / Role(s) |
| Cm R (rev) APr (rev) ccdB (rev) ccdA- (rev) BB-Pre BB-Post rep (pMB1) (rev) terminator terminator ccD Operon (rev) VF VR ORI VF2 | 1861,2519 2631,3491 52,357 359,579 3,18 701,719 959,1573 3703,3722 728,762 23,699 3534,3553 855,874 974,974 3614,3633 | CDS feature/cds CDS feature/cds feature/cds CDS feature/cds CDS feature/BioBrick engineered_region engineered_region feature/BioBrick sequence_feature feature/misc feature/stem_loop stem_loop feature/stem_loop stem_loop sequence_feature feature/misc primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding feature/misc sequence_feature feature/primer_binding primer_binding_site |