pSB1AC3-1

pSB1AC3_1 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:pSB1AC3-1
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-06 11:00:00
Date modified: 2015-05-08 01:14:55

pSB1AC3 with ccdB insert



Types
DnaRegion

Roles
Plasmid

plasmid

Sequences pSB1AC3_1_sequence (Version 1)

Description

Target plasmid for three antibiotic assembly. Chloramphenicol resistant. Contains an insert having a constitutive promoter and the ccdB coding region. ccdB protein is lethal in normal cloning strains. Grown in the special strain DB3.1, which is ccdB resistant.

Sequence Annotation Location Component / Role(s)
Cm R (rev)
APr (rev)
ccdB (rev)
ccdA- (rev)
BB-Pre
BB-Post
rep (pMB1) (rev)
terminator
terminator
ccD Operon (rev)
VF
VR
ORI
VF2
1861,2519
2631,3491
52,357
359,579
3,18
701,719
959,1573
3703,3722
728,762
23,699
3534,3553
855,874
974,974
3614,3633
CDS feature/cds
CDS feature/cds
feature/cds CDS
feature/cds CDS
feature/BioBrick engineered_region
engineered_region feature/BioBrick
sequence_feature feature/misc
feature/stem_loop stem_loop
feature/stem_loop stem_loop
sequence_feature feature/misc
primer_binding_site feature/primer_binding
primer_binding_site feature/primer_binding
feature/misc sequence_feature
feature/primer_binding primer_binding_site
igem#experience Works  
igem#sampleStatus Discontinued  
igem#status Deleted  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel pSB1AC3_1/1