Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Registry
Date created: 2004-07-27 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | pSB1AK3_sequence (Version 1) |
Description
pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).| Sequence Annotation | Location | Component / Role(s) |
| Suffix Prefix rep (pMB1) (rev) kanR (rev) ampR (rev) ORI terminator stem loop terminator stem loop VF2 VR deletion of two bases E. coli his terminator end of terminator | 1,21 3168,3189 261,875 1097,1912 2067,2927 276,276 3139,3158 30,64 3050,3069 157,176 34,35 22,93 3126,3129 | feature/BioBrick engineered_region feature/BioBrick engineered_region CDS feature/cds CDS feature/cds feature/cds CDS sequence_feature feature/misc stem_loop feature/stem_loop stem_loop feature/stem_loop feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc |