pSB1AK3-1

pSB1AK3_1 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:pSB1AK3-1
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2005-06-05 11:00:00
Date modified: 2015-05-08 01:14:55

PSB1AK3 with ccdB insert



Types
DnaRegion

Roles
Plasmid

plasmid

Sequences pSB1AK3_1_sequence (Version 1)

Description

Target plasmid for three antibiotic assembly. The ccdB insert prevents growth of the unmodified plasmid in normal cloning strains, allowing selection of only correctly assembled recombinants.

Sequence Annotation Location Component / Role(s)
kanR (rev)
ampR (rev)
ccdB (rev)
ccdA- (rev)
rep (pMB1) (rev)
BB-Pre
BB-Post
Vf
Vr
terminator
terminator
ORI
ccD Operon (rev)
VF2
1912,2727
2882,3742
169,474
476,696
1076,1690
120,135
818,836
3785,3804
972,991
90,109
845,879
1091,1091
140,816
1,20
feature/cds CDS
feature/cds CDS
CDS feature/cds
feature/cds CDS
feature/misc sequence_feature
feature/BioBrick engineered_region
engineered_region feature/BioBrick
primer_binding_site feature/primer_binding
primer_binding_site feature/primer_binding
feature/stem_loop stem_loop
stem_loop feature/stem_loop
feature/misc sequence_feature
feature/misc sequence_feature
feature/primer_binding primer_binding_site
igem#experience Works  
igem#sampleStatus Discontinued  
igem#status Deleted  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel pSB1AK3_1/1